多乐彩规则:Sinofection? transfection

江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Sinofection? is a superior cationic polymer-based transfection reagent. Compared with liposomes on the market, Sinofection exhibits lower cytotoxicity, higher transfection efficiency and higher repeatability.

Sinofection? Transfection Reagent

STF02: Superior cationic polymer-based; For DNA transfection, hEK293 cell and other cells' transfection

Sinofection? Transfection Reagent Feature

? For DNA transfection ? For eukaryotic cells like HEK293 ? For both adherent and suspension cells ? For operations with or without serum
? Inorganic reagent without animal ingredients ? More then 180000 L application ? Easy operation and less time taken ? Stable with strict quality control
? Higher transfection efficiency ? Lower cytotoxicity ? higher protein expression ? Suit for kinds of cells

High protein expression and low cytotoxicity(EGFP protein expression transfected Sinofection )

293E
293FT
COS7
HepG2
BHK21
MCF7
SW480
CHO-DG44

Sinofection? Transfection Reagent Suitable for Following Cell Types

? HEK293H ? MCF7 ? Hela ? A549
? HEK293FT ? SW480 ? Caco2 ? NIH/3T3
? HEK293E ? MDCK ? BHK21 ? Vero
? HEK293F ? CHO-K1 ? CHO-DG44 ? SF9
? COS7 ? HepG2 ? RAW264.7  

Usage of Sinofection in different cell lines(96-well plate)

Cell type Culture medium Cells per well DNA Sinofection Medium change after 4-6h
293H DMEM 3×104 0.2μg 0.5μL DMEM+10%FBS
293FT DMEM 3×104 0.2μg 0.5μL DMEM+10%FBS
293E DMEM 3×104 0.2μg 0.5μL DMEM+10%FBS
293F DMEM 3×104 0.2μg 0.5μL DMEM+10%FBS
COS7 DMEM 1.5×104 0.4μg 0.5μL DMEM+10%FBS
hela DMEM 2×104 0.3μg 0.5μL 1640+15%FBS
Caco2 MEM 3.5×104 0.3μg 0.75μL MEM+10%FBS
BHK21 MEM 2×104 0.2μg 0.5μL MEM+10%FBS
CHO-DG44 DMEM+HT+pro 2×104 0.5μg 0.5μL DMEM+HT+pro +10%FBS
RAW264.7 DMEM 3×104 0.2μg 0.5μL DMEM +10%FBS
MCF7 MEM/NEAA+0.01mg/mL insulin + sodium pyruvat 2×104 0.1μg 0.25μL MEM/NEAA+0.01mg/mL insulin + sodium pyruvat+10%FBS
SW480 IMDM 3×104 0.4μg 0.5μL IMDM +10%FBS
MDCK DMEM 4×104 0.6μg 1μL DMEM+10%FBS
CHO-K1 IMDM+Pro 3×104 0.2μg 0.5μL IMDM+Pro +10%FBS
HepG2 DMEM 3×104 0.5μg 0.75μL DMEM+10%FBS
A549 DMEM 2×104 0.3μg 0.5μL DMEM+10%FBS
NIH/3T3 DMEM 1.5×104 0.1μg 0.75μL DMEM+10%FBS
vero DMEM 3×104 0.3μg 0.75μL DMEM+10%FBS
sf9 SIM SF 5×104 0.4μg 0.75μL SIM SF+10%FBS