江西多乐彩杀号:Standard Mouse Monoclonal Antibody Production Service
Standard Mouse Mab Production Service Introduction
江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Mouse mAb has many features such as targeting to a single epitope, high specificity, stable passage, large-scale repeated production and extensive applications in basic research, medical diagnosis, tumor therapy and radioimmunoimaging. Mouse mAb developed by Sino Biological has the advantages of high sensitivity & specificity. We can also provide flexible customized solutions according to customer requirements, aiming to provide customers with high-quality antibodies.
More Antibody Production & Development ServicesQuote！
Standard Mouse Mab Production Service Process
| Service procedures | Specification | Timeline | Deliverables | Guarantee | Price |
|---|---|---|---|---|---|
| ① Antigen preparationQuote！ |
Please refer to antigen production service |
Please fill in the form and send it to [email protected] |
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| ② Antigen validation |
? Analysis of client's antigen by SDS-PAGE and UV | 1-2 days | ? 2-5 strains of ELISA positive clones (2 tubes of cryopreserved cells) ? 2-5mL supernatant per clone ? 1-3mg purified antibody (choose a hybridoma cell) ? Experimental report |
ELISA positive for immunogen | |
| ③ Immunization and serum titer test |
? Pre-immune bleed ? Five Balb/c mice immunization ? Serum titer test ? Final bleed |
8-10 weeks | |||
| ④ Fusion and Screening |
? Fusion and Screening ? Subcloning and cell expansion ? Cell Banking |
4-6 weeks | |||
| ⑤ Antibody production & purification |
? Appropriate scale hybridoma cell culture ? Purified by protein A affinity chromatography |
2-3 weeks | |||
| ⑥ QC analysis |
? Analysis by SDS-PAGE and UV ? ELISA validation |
3 days | |||
| Recombinant protein antigen | Peptide antigen | ||
|---|---|---|---|
| Antigen quantities | 3-4mg/5 mice | ? KLH/VLP conjugated peptides | 5mg/5 mice |
| Antigen Size? | >10kD | ? Concentration? | >0.5mg/mL? |
| SDS-Page purity? | >90% | ? OVA/Biotin conjugated peptides? | 2-3mg |
| Concentration? | >0.5mg/mL? | Concentration? | >0.1mg/mL? |
| Formulation? | PBS, if not PBS, please inquire first | Formulation | PBS, if not PBS, please inquire first |
Case Presentation of Standard Mouse Mab Production Service
Sino Biological Inc. has established a high-throughput mouse monoclonal antibody R&D platform. A set of high-throughput R&D programs for mouse monoclonal antibodies are also established through the optimization of immunization protocols, fusion protocols, screening protocols, and high-throughput antibody production and purification protocols. All the mouse Mab are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.
a）Multi-cell, multi-tissue validation
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? Human placenta 
Fig 1. Immunochemical staining of human target D with mouse Mab. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen. -
? Human breast carcinoma 
Fig 2. Immunochemical staining of human target D with mouse monoclonal antibody.
b）Multi-application verification
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Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane.
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Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. -
Fig 3. Lane A: Jurkat Whole Cell Lysate. Anti-target D mouse Mab; goat Anti-mouse IgG H&L (Dylight800).
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Fig 4. Lane A: Hela Whole Cell Lysate; Lane B: Jurkat Whole Cell Lysate; Lane C: Daudi Whole Cell Lysate. Anti-target D mouse Mab; Dylight 800-labeled antibody to Mouse IgG (H+L).
Standard Mouse Mab Production Service Advantages
- International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression.
- Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%.
- Customized immunization, screening and detection proposed according to customer's final application requirements can be provided.
- ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity.
- Quoting by steps, charging by nodes, reducing customer's risk and saving costs.
- Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion.
