江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Mouse mAb has many features such as targeting to a single epitope, high specificity, stable passage, large-scale repeated production and extensive applications in basic research, medical diagnosis, tumor therapy and radioimmunoimaging. Mouse mAb developed by Sino Biological has the advantages of high sensitivity & specificity. We can also provide flexible customized solutions according to customer requirements, aiming to provide customers with high-quality antibodies.
Service procedures | Specification | Timeline | Deliverables | Guarantee | Price |
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① Antigen preparationQuote! |
Please refer to antigen production service |
Please fill in the form and send it to [email protected] |
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② Antigen validation |
? Analysis of client's antigen by SDS-PAGE and UV | 1-2 days | ? 2-5 strains of ELISA positive clones (2 tubes of cryopreserved cells) ? 2-5mL supernatant per clone ? 1-3mg purified antibody (choose a hybridoma cell) ? Experimental report |
ELISA positive for immunogen | |
③ Immunization and serum titer test |
? Pre-immune bleed ? Five Balb/c mice immunization ? Serum titer test ? Final bleed |
8-10 weeks | |||
④ Fusion and Screening |
? Fusion and Screening ? Subcloning and cell expansion ? Cell Banking |
4-6 weeks | |||
⑤ Antibody production & purification |
? Appropriate scale hybridoma cell culture ? Purified by protein A affinity chromatography |
2-3 weeks | |||
⑥ QC analysis |
? Analysis by SDS-PAGE and UV ? ELISA validation |
3 days |
Recombinant protein antigen | Peptide antigen | ||
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Antigen quantities | 3-4mg/5 mice | ? KLH/VLP conjugated peptides | 5mg/5 mice |
Antigen Size? | >10kD | ? Concentration? | >0.5mg/mL? |
SDS-Page purity? | >90% | ? OVA/Biotin conjugated peptides? | 2-3mg |
Concentration? | >0.5mg/mL? | Concentration? | >0.1mg/mL? |
Formulation? | PBS, if not PBS, please inquire first | Formulation | PBS, if not PBS, please inquire first |
Sino Biological Inc. has established a high-throughput mouse monoclonal antibody R&D platform. A set of high-throughput R&D programs for mouse monoclonal antibodies are also established through the optimization of immunization protocols, fusion protocols, screening protocols, and high-throughput antibody production and purification protocols. All the mouse Mab are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.
a)Multi-cell, multi-tissue validation
? Human placenta |
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Fig 1. Immunochemical staining of human target D with mouse Mab. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen. |
? Human breast carcinoma |
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Fig 2. Immunochemical staining of human target D with mouse monoclonal antibody. |
b)Multi-application verification
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Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane. |
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Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
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Fig 3. Lane A: Jurkat Whole Cell Lysate. Anti-target D mouse Mab; goat Anti-mouse IgG H&L (Dylight800). |
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Fig 4. Lane A: Hela Whole Cell Lysate; Lane B: Jurkat Whole Cell Lysate; Lane C: Daudi Whole Cell Lysate. Anti-target D mouse Mab; Dylight 800-labeled antibody to Mouse IgG (H+L). |