江西多乐彩开奖号码:Custom Recombinant Protein Expression Services

Why choose us for protein production service ?

? Over 10 years' experience for 6000+ recombinant proteins production.
? One-stop service from gene to protein.
? Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
? Multiple purification systems (30+) to choose.
? Abundant R & D ability with over 1000 new proteins per year and quick troubleshooting.
? Close to 100 bioreactors with various volumes ranging from 2 to 1000 L to meet high-throughput and large-scale production.

NOTE: This video displays in Chinese.

All protein production service

  • ? Gene synthesis and codon optimization (optional)
    ? Vector construction
    ? Expression evaluation
    ? Protein expression and purification
    ? Large-scale expression and purification (optional)
  • ? Gene synthesis and codon optimization (optional)
    ? Vector construction
    ? Expression and purification
    ? Large-scale expression and purification (optional)
  • ? Gene synthesis and codon optimization (optional)
    ? Vector construction
    ? Virus packaging and amplification
    ? Expression and purification
    ? Large-scale expression and purification (optional)

Case Studies of Recombinant Protein Production Service

江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Case study of E.coli protein A expression     More details>>>

  • SEC-HPLC >98.5%
  • Cell bioactivity: EC50=17pg/mL

Case study of Baculovirus-Insect protein A expression     More details>>>

  • SEC-HPLC >93%
  • Cell bioactivity: EC50=7ng/mL

Case study of HEK293 transient protein A expression     More details>>>

  • SEC-HPLC >98.2%
  • SDS-PAGE >98.7%

Custom Recombinant Protein Production Service Advantages

E.coli protein expression service ? Over 10 years' experience of protein expression
? Multiple protein purification and refolding technologies
? High-throughput and large-scale protein expression and production
? One-stop service from gene to purified protein
? Multiple expression vectors and E.coli cells to meet various expression needs
Mammalian transient protein expression service ? High-efficiency expression vectors
? Optimized mammalian expression systems
? High-throughput and large-scale production
? High yield expression with post-translational modification and correct folding
? One-stop service from gene sequence to purified protein
Baculovirus-Insect Cell Protein Expression Service ? High-efficiency expression of intracellular and extracellular proteins
? Multiple insect cells: Sf9,Sf21,Hi-5
? High-throughput and large-scale protein production
? Optimized expression vectors and higher-titre virus packaging

Recombinant Protein Production Systems Reference

Expression systems E.coli Yeast Baculovirus-insect Mammalian
prokaryotic eukaryotic eukaryotic eukaryotic
Advantages ? low cost
? rapid expression
? easy scale-up
? most widely used system for recombinant protein production
? low cost
? rapid expression
? high yield
? diverse post-translational modifications
? high capacity genes
? soluble proteins
? suitable for toxic proteins
? post-translational modifications similar to mammalian systems
? soluble protein
? lower endotoxin
? better bioactivity
? comprehensive post-translational modifications
? transient & stable expression
Challenges ? inclusion bodies
? lack of eukaryotic post-translational modifications
? difficult to express higher MW proteins
? improper glycosylation
? high mannose modification
? more demanding culture conditions
? lack of partial glycosylation
? more demanding culture conditions
Most common applications ? bacterial proteins
? antigen proteins
? cytokines
? enzymes
? cytokines
? lower MW proteins
? enzymes
? cytoplasmic proteins
? toxic proteins
? transmembrane proteins
? secretory proteins
? kinases
? secretory proteins
? extracellular domain of transmembrane proteins
? recombinant antibodies
? antibody fragments

Contact Us

Tel
+86-10-58628216; +86-10-58628223