江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Sino Biological Inc. can achieve rapid and high-throughput recombinant antibody production. The preparation cycle is only 2-6 weeks, and the monomer purity can reach 99%.
? SDS-PAGE | ? SEC-HPLC >99% |
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Sino Biological Inc. has established a high-throughput mouse & rabbit monoclonal antibody R&D platform, it also has rich experience of rabbit polyclonal antibody development. All the mAb or Pab are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.
a)Multi-cell, multi-tissue validation |
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Fig 1. Immunochemical staining of human target E in human stomach with rabbit Mab. The image showing membrane staining of epithelium cell. |
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Fig 2. Immunochemical staining of human target E in human gastric cancer with rabbit Mab. The image showing membrane staining of epithelium cell. |
b)Multi-application verification |
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Fig 1. Immunofluorescence staining of human target E in HeLa cells with rabbit Mab. The image showing membrane staining of HeLa cells. |
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Fig 2. Lane A: A431 Whole Cell Lysate. Anti-target E rabbit Mab; Goat Anti-Rabbit IgG H&L (Dylight800). |
a)Multi-cell, multi-tissue validation |
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Fig 1. Immunochemical staining of human target D in human breast carcinoma with mouse Mab. |
b)Multi-application verification |
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Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane. |
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Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
a)Multi-cell, multi-tissue validation |
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Fig 1. Immunochemical staining of mouse target A in mouse liver with rabbit Pab. |
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Fig 2. Immunochemical staining of mouse target A in mouse kidney with rabbit Pab. |
b)Multi-application verification |
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Fig 1. Immunofluorescence staining of mouse target A in NIH-3T3 cells. Positive staining was localized to Mitochondrion. |
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Fig 2. Lane A: NIH-3T3 Whole Cell Lysate; Lane B: Raw264.7 Whole Cell Lysate. Anti-target A rabbit Pab; Goat Anti-Rabbit IgG (H+L)/HRP. |
Antibody Production | ? Recombinant antibody production is a key technology for the industrialization of monoclonal antibody drugs. Without immunization and screening process, antibody gene sequence can be directly & stably used to produce large amounts of antibodies. Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers. |
Rabbit Mab Production Service | ? International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression |
? Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95% | |
? Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies | |
? ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity | |
? High affinity, 10-100 times more than mouse mAb, in average |
Standard Mouse Mab Production Service | ? Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95% |
? Customized immunization, screening and detection proposed according to customer's final application requirements can be provided | |
? ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity | |
? Quoting by steps, charging by nodes, reducing customer's risk and saving costs | |
? Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion |
Standard Rabbit Pab Production Service | ? International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression |
? Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95% | |
? Serum titer ≥1:64,000 | |
? Quoting by steps, charging by nodes, reducing customer's risk and saving costs |
Speedy Rabbit Pab Production Service | ? World-leading recombinant protein expression and purification techniques, professional peptide design and high-efficiency conjugation techniques |
? Short production time, completing antibody production within 45 days | |
? Guaranteed Rabbit pAb with a serum ELISA titer of ≥1:64,000 | |
? Antibody with high sensitivity, using less antibody each time | |
? Guaranteed delivery amount, meeting experiment needs | |
? Quoting by steps, charging by nodes, reducing customer's risk and saving costs |
Speedy Mouse Mab Production Service | ? Short production time for antibodies |
? Without increasing the dose of immunogens | |
? High fusion efficiency with electrofusion technique | |
? Able to produce antibodies for specific application such as FC, IHC and WB | |
? Rapid production scale from milligram to gram level | |
? Antibody with high purity, high lot-to-lot reproducibility and without animal viruses contamination |
Antigen Production Service | ? International leading recombinant protein production and purification technology, with five antigen expression platforms: HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification |
? With intellectual property of medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost | |
? The leading technical personnel and laboratory equipment, rich experience on 6000+ protein expression and purification, supporting high-throughput, large-scale and rapid production | |
? Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95% |
ELISA Kit development Service | ? Abundant resources: Sino Biological has developed nearly 30,000 antibodies covering 2,200 targets and has a protein library with >6000 proteins; We have 5 major protein expression and production platforms including HEK293, CHO, E.coli, Yeast, and Insect cell, As well as optimized reagent formulation, enzyme labeling technology and production process. Sino Biological can also customize related products and carry out kit development according to customers' needs in least time. |
? Professional R&D platform: Sino Biological has developed more than 100,000 ELISA antibody in the past 10 years. We have successfully developed 400+ ELISA pair set and 150+ ELISA kits. Sino Biological also has rich experience on ELISA kit customization for dozens of biopharmaceutical companies. | |
? Professional technical support: Our techincal support team will provide suitable proposal to meet customers' need fastly and efficiently. | |
? Quality assurance: We have various natural samples, as well as testing experience which can be used for comprehensive and accurate methodological validation of Elisa kits. | |
? Convenient and fast: We can provide one-stop service with less time lines and high efficiency, which can help customer save time |
Items | Recombinant antibody | Traditional antibody |
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Timeline | Short cycle, only 2-6 weeks | Longer cycle, 4-6 months |
Antibody forms | The form is not limited. It can be genetically engineered at the genetic level to custom antibodies to different requirements, for example, fragment antibody scFv and Fab, full-length antibody, bispecific antibody, etc. site-directed mutagenesis (variable and constant regions transformation), cross-subtype (IgG1-IgG4), and cross-species (Human/mouse chimeric antibodies, etc.) | Single form: full-length antibodies limited to fixed species |
Production pattern | Extremely flexible. It can be produced in small scale production or in high-throughput mass production. | Not flexible. Production pattern are subject to certain restrictions depending on the needs. |
Lot-to-lot consistency | High lot-to-lot consistency. The production of recombinant antibodies is a standardized process with controllable, traceable and reproducible results. Recombinant antibodies are a better choice for other assays such as ELISA, IHC and Western Blotting. | There is a certain degree of inter-batch difference in antibodies based on individual reasons and immunizations. The inter-batch differences of monoclonal antibodies are controllable, while that of the polyclonal antibodies are generally uncontrollable. |
Antibody labeling | Enzyme labeling, fluorescent labeling, isotope labeling, biotin labeling, etc. | Enzyme labeling, fluorescent labeling, isotope labeling, biotin labeling, etc. |
Immune animal | No | Immunization of animals cannot be completely eliminated to obtain antibodies |
Application | Detection antibody, diagnostic reagents, antibody drug | Detection antibody, diagnostic reagents |