江西多乐彩登陆:Custom Antibody Services (Production & Development)

Custom Antibody Production & Development Service Introduction

Sino Biological has more than 10 years experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma antibody gene sequencing, chimeric antibody and antibody fragment expression, antibody humanization, antibody affinity maturation, anti-idiotype antibody and bispecific antibody production services.
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Most Popular Antibody Production Service

More Antibody Production & Development Service

  • ? Gene synthesis and codon optimization (optional)
    ? Vector construction
    ? Expression & purification
    ? QC analysis
  • ? Antigen preparation
    ? Antigen validation
    ? Immunization and serum titer test
    ? Library construction & screening
    ? Antibody production & purification
    ? QC analysis

Case Presentation of Antibody Production & Development Service

A）Recombinant antibody production：

江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Sino Biological Inc. can achieve rapid and high-throughput recombinant antibody production. The preparation cycle is only 2-6 weeks, and the monomer purity can reach 99%.

? SDS-PAGE ? SEC-HPLC >99%

B）Recombinant antibody development：

Sino Biological Inc. has established a high-throughput mouse & rabbit monoclonal antibody R&D platform, it also has rich experience of rabbit polyclonal antibody development. All the mAb or Pab are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.

? Rabbit Mab development service

  • a）Multi-cell, multi-tissue validation
    Fig 1. Immunochemical staining of human target E in human stomach with rabbit Mab. The image showing membrane staining of epithelium cell.
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    Fig 2. Immunochemical staining of human target E in human gastric cancer with rabbit Mab. The image showing membrane staining of epithelium cell.
  • b）Multi-application verification
    Fig 1. Immunofluorescence staining of human target E in HeLa cells with rabbit Mab. The image showing membrane staining of HeLa cells.
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    Fig 2. Lane A: A431 Whole Cell Lysate. Anti-target E rabbit Mab; Goat Anti-Rabbit IgG H&L (Dylight800).

? Standard Mouse Mab development service

  • a）Multi-cell, multi-tissue validation
    Fig 1. Immunochemical staining of human target D in human breast carcinoma with mouse Mab.
  • b）Multi-application verification
    Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane.
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    Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

? Standard Rabbit Pab development service

  • a）Multi-cell, multi-tissue validation
    Fig 1. Immunochemical staining of mouse target A in mouse liver with rabbit Pab.
  •  
    Fig 2. Immunochemical staining of mouse target A in mouse kidney with rabbit Pab.
  • b）Multi-application verification
    Fig 1. Immunofluorescence staining of mouse target A in NIH-3T3 cells. Positive staining was localized to Mitochondrion.
  •  
    Fig 2. Lane A: NIH-3T3 Whole Cell Lysate; Lane B: Raw264.7 Whole Cell Lysate. Anti-target A rabbit Pab; Goat Anti-Rabbit IgG (H+L)/HRP.

Antibody Production & Development Service Advantages

Antibody Production ? Recombinant antibody production is a key technology for the industrialization of monoclonal antibody drugs. Without immunization and screening process, antibody gene sequence can be directly & stably used to produce large amounts of antibodies. Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.
Rabbit Mab Production Service ? International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression
? Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
? Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies
? ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
? High affinity, 10-100 times more than mouse mAb, in average
Standard Mouse Mab Production Service ? Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
? Customized immunization, screening and detection proposed according to customer's final application requirements can be provided
? ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
? Quoting by steps, charging by nodes, reducing customer's risk and saving costs
? Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion
Standard Rabbit Pab Production Service ? International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression
? Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
? Serum titer ≥1:64,000
? Quoting by steps, charging by nodes, reducing customer's risk and saving costs
Speedy Rabbit Pab Production Service ? World-leading recombinant protein expression and purification techniques, professional peptide design and high-efficiency conjugation techniques
? Short production time, completing antibody production within 45 days
? Guaranteed Rabbit pAb with a serum ELISA titer of ≥1:64,000
? Antibody with high sensitivity, using less antibody each time
? Guaranteed delivery amount, meeting experiment needs
? Quoting by steps, charging by nodes, reducing customer's risk and saving costs
Speedy Mouse Mab Production Service ? Short production time for antibodies
? Without increasing the dose of immunogens
? High fusion efficiency with electrofusion technique
? Able to produce antibodies for specific application such as FC, IHC and WB
? Rapid production scale from milligram to gram level
? Antibody with high purity, high lot-to-lot reproducibility and without animal viruses contamination
Antigen Production Service ? International leading recombinant protein production and purification technology, with five antigen expression platforms： HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification
? With intellectual property of medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost
? The leading technical personnel and laboratory equipment, rich experience on 6000+ protein expression and purification, supporting high-throughput, large-scale and rapid production
? Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95%
ELISA Kit development Service ? Abundant resources: Sino Biological has developed nearly 30,000 antibodies covering 2,200 targets and has a protein library with >6000 proteins; We have 5 major protein expression and production platforms including HEK293, CHO, E.coli, Yeast, and Insect cell, As well as optimized reagent formulation, enzyme labeling technology and production process. Sino Biological can also customize related products and carry out kit development according to customers' needs in least time.
? Professional R&D platform: Sino Biological has developed more than 100,000 ELISA antibody in the past 10 years. We have successfully developed 400+ ELISA pair set and 150+ ELISA kits. Sino Biological also has rich experience on ELISA kit customization for dozens of biopharmaceutical companies.
? Professional technical support: Our techincal support team will provide suitable proposal to meet customers' need fastly and efficiently.
? Quality assurance: We have various natural samples, as well as testing experience which can be used for comprehensive and accurate methodological validation of Elisa kits.
? Convenient and fast: We can provide one-stop service with less time lines and high efficiency, which can help customer save time

Antibody Production & Development Service Reference

Items Recombinant antibody Traditional antibody
Timeline Short cycle, only 2-6 weeks Longer cycle, 4-6 months
Antibody forms The form is not limited. It can be genetically engineered at the genetic level to custom antibodies to different requirements, for example, fragment antibody scFv and Fab, full-length antibody, bispecific antibody, etc. site-directed mutagenesis (variable and constant regions transformation), cross-subtype (IgG1-IgG4), and cross-species (Human/mouse chimeric antibodies, etc.) Single form: full-length antibodies limited to fixed species
Production pattern Extremely flexible. It can be produced in small scale production or in high-throughput mass production. Not flexible. Production pattern are subject to certain restrictions depending on the needs.
Lot-to-lot consistency High lot-to-lot consistency. The production of recombinant antibodies is a standardized process with controllable, traceable and reproducible results. Recombinant antibodies are a better choice for other assays such as ELISA, IHC and Western Blotting. There is a certain degree of inter-batch difference in antibodies based on individual reasons and immunizations. The inter-batch differences of monoclonal antibodies are controllable, while that of the polyclonal antibodies are generally uncontrollable.
Antibody labeling Enzyme labeling, fluorescent labeling, isotope labeling, biotin labeling, etc. Enzyme labeling, fluorescent labeling, isotope labeling, biotin labeling, etc.
Immune animal No Immunization of animals cannot be completely eliminated to obtain antibodies
Application Detection antibody, diagnostic reagents, antibody drug Detection antibody, diagnostic reagents

Custom Antibody Production & Development Service Features

  • Varied expression platforms for antigen: Five antigen expression platforms including HEK293, CHO, E.coli, Yeast,and Insect cell can meet the needs of different types of antigen recombinant expression.
  • Multiple antigen types: Polypeptide and protein antigens are two main methods for immunization and screening, various methods such as DNA, cell and insect virus immunization are also available.
  • Various animal species for immnization: Mouse and rabbit.
  • Different antibody development technologies: Hybridoma technology and antibody library screening technology can be used to increase the probability of obtaining positive clones and high-specificity & high-affinity antibodies.
  • Diversified antibody screening technologies: Solid-phase and liquid-phase antibody screening techniques can both be used to reduce the amount of coating antigen and increase antibody diversity.
  • Multiple antibody detection platforms: ELISA, WB, IHC, FACS, SEC-HPLC, Octet, endotoxin, mass spectrometry and other antibody detection platforms can be used for comprehensive assessment of antibody quality.
  • Various antibody purification methods: Protein A, protein G, protein L, size exclusion chromatography, ion exchange chromatography, antigen affinity purification and other purification methods will be done to improve antibody purity.
  • Antibody recombinant expression technology: Using optimized plasmids and HEK293 cell line, Sino Biological enables rapid, high-throughput and large-scale production of recombinant antibodies and antibody fragments.
  • Hybridoma culture technology: Serum-free suspension culturing of hybridoma cells can be used, compared with ascites, the antibody will not include complex components and will avoid the serum influence on detection.

Contact Us

Tel
+86-10-58628216; +86-10-58628223
Email
[email protected]