多乐彩任选5技巧:Baculovirus-Insect Cell Expression Service
Baculovirus-Insect Cell Protein Expression Service Introduction
江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn Baculovirus-insect cell system belongs to eukaryotic expression system, with capability of protein folding and modification after protein translation. It is suitable for expressing proteins that are probably harmful to mammalian host cells, such as kinases and toxic proteins. Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.
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Baculovirus-Insect Cell Protein Expression Service Items
| Service procedures | Specification | Timeline | Deliverables | Pricing |
|---|---|---|---|---|
| ① Gene synthesis and codon optimization (optional) |
1-2 weeks | ? Sequencing report |
Please fill in the form and send it to [email protected] |
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| ② Vector construction |
? Clone cDNA into expression vector ? Plasmid sequencing ? Large-scale plasmid preparation |
1-2 weeks | ||
| ③ Virus packaging and amplification |
? Virus packaging ? Virus amplification |
2-3 weeks | ||
| ④ Expression and purification |
? Virus infecting cells ? Small expression, purification and condition optimization ? SDS-PAGE and UV analysis |
2-3 weeks | ? Feasibility report ? 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing) |
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| ⑤ Large-scale expression and purification (optional) |
2-3 weeks | ? Purified protein ? SDS-PAGE report ? CoA |
Baculovirus-Insect Cell Protein Expression Service Procedures
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| Communicate about protein expression requirements | ? | Sign contract and make prepayment | ? | Carry out pilot study for protein expression | ? | Feedback pilot study result and ship sample (if feasible) | ? | Bulk production quotation | ? | Bulk production based on customer requirements |
Case Presentation of Baculovirus-Insect Cell Protein Expression Service
Protein A:
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1. SDS-PAGE >97% 
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2. SEC-HPLC >93% 
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3. Cell-based activity assay：EC50=7ng/mL 
Baculovirus-Insect Cell Protein Expression Service Advantages
- Over 10 years' experience of protein expression
- One-stop service from gene sequence to purified protein
- High-efficiency expression of intracellular and extracellular proteins
- Multiple insect cells: Sf9, Sf21, Hi-5
- High-throughput and large-scale protein production
- Optimized expression vectors and higher-titer virus packaging
Contact Us
Tel
+86-10-58628216; +86-10-58628223
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