江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn The mouse antibody has a strong immune response to humans and can induce a human anti-mouse antibody response (HAMA response), which affects the safety and therapeutic effect of the mouse Mab in clinical application. Mouse antibodies can be genetically modified to obtain a structure similar to that of human antibodies, so as to avoid recognition by the immune system and avoid HAMA. Sino Biological Inc. uses CDR grafting technology and computer-aided structural modeling to humanize mouse Mab and rabbit Mab to ensure humanization >90%. Compared with the original mouse antibody or chimeric antibody, the affinity is substantially unchanged, and techniques such as antibody affinity maturation can be provided to further increase the affinity of the humanized antibody. Learn more about "antibody affinity maturation service".
Service procedures | Specification | Timeline | Deliverables | Pricing |
---|---|---|---|---|
① Antibody humanization design |
? Antibody sequence analysis ? Antibody 3D structure modeling ? CDR structural analysis ? Humanized mutant design |
1-2 weeks | ? 3 strains of purified humanized antibody with the best affinity, 0.2~0.5mg per strain ? Humanized antibody sequences ? Vectors containing H and L chain, respectively ? CoA |
Please fill in the form and send it to [email protected] |
② Gene synthesis and codon optimization |
1-2 weeks | |||
③ Vector construction |
? Cloning the HC and LC gene into optimized expression vectors, respectively ? Plasmid sequencing ? Large scale plasmid production |
1-2 weeks | ||
④ Expression & purification |
? Transient transfection of HEK293 cells ? Antibody purification ? Analysis by SDS-PAGE and UV |
2-3 weeks | ||
⑤ Affinity analysis |
? ELISA and Octet | 1-2 weeks |