江西多乐彩11选5:TARC ELISA Kit, Human

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TARC ELISA Kit, Human: General Information

Product name
TARC ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
9.45 pg/mL
Assay range
18.75-1200 pg/mL
Specificity
Recognizes both recombinant and natural Human TARC
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 65.00 178.00 449.00
SD 6.26 13.25 9.02
CV(%) 9.7% 7.5% 2.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 142.00 301.00 626.00
SD 13.87 17.67 62.01
CV(%) 9.7% 5.9% 9.9%
Recovery
The recovery of Human CCL17 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Serum
  Recovery of detected
1:2 98%
1:4 92%
1:8 110%
1:16 120%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TARC ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TARC protein in Serum . It contains recombinant Human TARC, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TARC ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CCL17. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TARC ELISA Kit, Human: Alternative Names

A-152E5.3 ELISA Kit, Human; ABCD-2 ELISA Kit, Human; SCYA17 ELISA Kit, Human; TARC ELISA Kit, Human

TARC Background Information

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokin ligand 17 (CCL17), also known as thymus and activation regulated chemokine(TARC), is a small cytokine belonging to the C-C chemokine family. CCL17 is expressed maily in thymus and transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. CCL17 can induce chemotaxis in T cells by binding with the chemokine receptor CCR4.
Full Name
chemokine (C-C motif) ligand 17
References
  • Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28(5): 443-60.
  • Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811-5.
  • Morita A, et al. (2002). Evaluation of human thymus and activation-regulated chemokine concentrations in blood using a new sandwich ELISA based on monoclonal antibodies. Clin Chim Acta. 322 (1-2): 67-75.
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