多乐彩脱胆怎么玩:Anti-V5 tag Antibody

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Anti-V5 tag Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-V5 tag Antibody
Validated applications
WB,IP
Specificity
V5 tag
Immunogen
A synthetic peptide corresponding to the V5 tag sequence.
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the V5-tag sequence. V5-tag specific IgG was purified by V5-taq affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-V5 tag Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:1000-1:5000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-V5 tag Antibody (Rabbit Polyclonal antibody) Images

江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn V5-tag was immunoprecipitated using:

Lane A:0.5 mg his-ARG1-V5 Transfected 293 Cell Lysate

Lane B:0.5 mg V5-ARG1-his Transfected 293 Cell Lysate

Lane C:0.5 mg mFABP4-V5-his Transfected 293 Cell Lysate

Lane D:0.5 mg V5-mFABP4-his Transfected 293 Cell Lysate

Lane E:0.5 mg Negative control

4 μL anti-V5-tag rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-V5-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution

Sample:Recombinant Protein

Lane A: V5-mFABP4-his(30ng)

Lane B: his-ARG1-V5(30ng)

Lane C: mFABP4-V5-his(30ng)

Lane D:Negative control

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution

Lane A: his-ARG1-V5 Transfected 293 Cell Lysate

Lane B: V5-ARG1-his Transfected 2933 Cell Lysate

Lane C: V5-mFABP4-his Transfected 293 Cell Lysate

Lane D: mFABP4-V5-his Transfected 293 Cell Lysate

Lane E: Negative control

Lysates/proteins at 2 μg per Lane.

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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