江西11选5(多乐彩:Anti-V5 tag Antibody
Cat: 100378-T36
Anti-V5 tag Antibody (Rabbit Polyclonal antibody) General Information
Anti-V5 tag Antibody (Rabbit Polyclonal antibody) Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| IP | 1-4 μl/mg of lysate |
Anti-V5 tag Antibody (Rabbit Polyclonal antibody) Images
-HB08JU2022-IP.png)
江西体彩11选5多乐彩走势图今天 www.phcnlx.com.cn V5-tag was immunoprecipitated using:
Lane A:0.5 mg his-ARG1-V5 Transfected 293 Cell Lysate
Lane B:0.5 mg V5-ARG1-his Transfected 293 Cell Lysate
Lane C:0.5 mg mFABP4-V5-his Transfected 293 Cell Lysate
Lane D:0.5 mg V5-mFABP4-his Transfected 293 Cell Lysate
Lane E:0.5 mg Negative control
4 μL anti-V5-tag rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-V5-tag rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution
Developed using the Odyssey technique.
Performed under reducing conditions.
-HB08JU2022-WB.png)
Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution
Sample:Recombinant ProteinLane A: V5-mFABP4-his(30ng)
Lane B: his-ARG1-V5(30ng)
Lane C: mFABP4-V5-his(30ng)
Lane D:Negative control
Secondary
Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.
-pAb(A)-HB08JU2022-WB.png)
Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution
Lane A: his-ARG1-V5 Transfected 293 Cell Lysate
Lane B: V5-ARG1-his Transfected 2933 Cell Lysate
Lane C: V5-mFABP4-his Transfected 293 Cell Lysate
Lane D: mFABP4-V5-his Transfected 293 Cell Lysate
Lane E: Negative control
Lysates/proteins at 2 μg per Lane.
Secondary
Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recombinant Antibody Production Service
High-throughput Recombinant Antibody Production
